Upgrade of BL-5C as a highly automated macromolecular crystallography beamline at Pohang Light Source II

BL-5C is an in-vacuum undulator beamline dedicated to macromolecular crystallography (MX) at the 3 GeV Pohang Light Source II in Korea. The delivers X-ray beams with a focal spot size of 200 µm × 40 (FWHM, H V) over energy range 6.5–16.5 keV. measured flux 7 10 11 photons s −1 12.659 keV through aperture 50 µm. experimental station newly equipped photon-counting detector EIGER 9M, multi-axis micro-diffractometer MD2, and robotic sample changer high-capacity dewar. These instruments enable operation this as automated MX specialized fragment screening. This can collect more than 400 data sets day without human intervention, difference map be automatically calculated by using processing pipeline for ligand or identification.